Figure 7.

Circadian, but not diurnal, expression patterns of CESAs are FKF1 dependent. One-week-old Arabidopsis seedlings grown under long-day conditions (16-h light/8-h dark) were transferred to continuous light conditions (24-h light) for 1 d. The seedlings were then harvested at 6-h intervals for 3 d (24–90 h) for expression analysis. To avoid variation, a total of 20 seedlings were pooled and used for gene expression analysis. This experiment was repeated three independent times. Data shown was an average of two technical replicates of three biological replicates. Error bars represent sd (n = 6). The gray rectangles indicate the subjective nighttime under continuous light experiment.