Profiling of SA contents in mutant lines and overexpression lines after treatment with MeSA. A, HPLC profiling of free SA levels in upper leaves of wild type, mutant lines (ko-1 and ko-2), and overexpression lines (OE-6 and OE-10) 48 h after inoculation of lower leaves with 1 mg/L MeSA. B, Quantification of free SA levels in upper leaves of wild type, mutant lines, and overexpression lines 48 h after inoculation of lower leaves with 1 mg/L MeSA or DMSO solvent (Mock). C, HPLC profiling of total SA levels in upper leaves of wild type, mutant lines (ko-1 and ko-2), and overexpression lines (OE-6 and OE-10) 48 h after inoculation of lower leaves with 1 mg/L MeSA. D, Quantification of total SA levels in upper leaves of wild type, mutant lines, and overexpression lines 48 h after treatment of lower leaves with 1 mg/L MeSA or DMSO solvent (Mock). “St” represents the authentic standard of SA. All experiments were repeated three times with similar results. Data = means ≥ sd (Student’s t test, **P < 0.01). WT, wild type.