Figure 6.

The var2-9 mutant may accumulate SA in the absence of transcriptional reprogramming of genes involved in SA accumulation. A and B, Results of RT-qPCR (A) and RNA-seq (B) analyses show no noticeable changes of the transcript levels of ICS1, EDS1, and PAD4 in the var2-9 seedlings compared with the wild type (WT) and var2-9 cpNahG (v/N). For the RT-qPCR, PP2A was used as an internal control, and the data represent means of three independent biological replicates. Error bars indicate sd. Lowercase letters in A indicate statistically significant differences between mean values (P < 0.05, one-way ANOVA with posthoc Tukey’s HSD test). C, Transcript levels of three genes (PAL1, PAL2, and PAL3) encoding PAL in wild-type and var2-9 plants were obtained by RNA-seq analysis. Error bars indicate sd (n = 3).