Figure 5.

Phenotypes of PuHSFA4a-OE, PuHSFA4a-SRDX dominant repressor, and wild-type (WT) P. ussuriensis plants after transplantation in soil under excess Zn. A, Phenotypic comparison of PuHSFA4a transgenic and wild-type plants with respect to above-ground parts, leaves, and roots after irrigation with 5.0 mm of ZnSO₄ for four weeks. Scale bars = 5 cm. B and C, Root dry weight (B) and plant height (C) of A4a-OE3, A4a-OE8, A4a-SRDX1, A4a-SRDX6, and wild-type P. ussuriensis plants in response to excess Zn. Each value represents the mean of 40 plants, and error bars = sd. D, Chlorophyll content of leaves of PuHSFA4a transgenic and wild-type plants with or without excess Zn treatment. E, Zn content of PuHSFA4a transgenic and wild-type plants roots under excess Zn treatment. Two-month–old plants were treated with 5.0 mm of ZnSO₄ for four weeks. Data are presented as means of three biological replicates, and error bars = sd. Asterisks indicate significant differences as determined by a Student’s t test, *P < 0.05 and **P < 0.01.