Figure 9.

Phenotype of PuPLA₂-OE and wild-type (WT) P. ussuriensis plants with or without Zn treatment. A, Phenotypic comparison of the roots of PuPLA₂ transgenic and wild-type plants with or without excess Zn. Scale bars = 2 cm. B and C, Root dry weight (B) and plant height (C) of PuPLA₂-OE and wild-type plants under excess Zn stress. Each value represents the mean of 30 plants, and error bars = sd. D, The relative expression levels of PuPIF3 in PuPLA₂-OE and wild-type plants assessed under excess Zn conditions, and a comparison of the relative expression of PuPIF3 in PuPLA₂-OE lines compared to wild-type plants. All values are normalized to PuActin (MH644084). Data are presented as means of three biological replicates, and error bars = sd. For (B)–(D), Asterisks indicate significant differences as determined by a Student’s t test, *P < 0.05 and **P < 0.01.