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. 2019 Jul 31;13:65. doi: 10.1186/s13036-019-0195-y

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Substrate specificity of MT_BSLS constructs. a Various MT_BSLS constructs that were evaluated. b Comparison of the preference of different MT_BSLS constructs to L-Phe-SNAC and L-Leu-SNAC. The ratio of methylated products formed from 5 mM L-Phe-SNAC or 5 mM L-Leu-SNAC over a 10-min reaction time are plotted. The data for cMT and XLcMT represent two separate preparations of crosslinked protein, assayed in duplicate