Fig. 6.

Piscidin protects mice from PRV-induced death. a. processed brain sections from control, co-treated, and PRV infected groups were subjected to Haematoxylin and Eosin (H&E). The representative H&E results were shown. b. The mice injected with piscidin or PRV, co-treated with piscidin and PRV were divided into different groups. Surviving mice were challenged with PRV on day 14 again. Survival status of control and experiment groups were monitored on a daily basis for 28 days (n = 10). In the group of piscidin (10 μg/ml), 2 mice were heavily infected and died on day 19 and 20. And another 5 mice with severe neurological symptoms were euthanized for animal welfare reasons; In the group of piscidin (0 μg/ml) + PRV, 2 mice were heavily infected and died on day 5. And another 7 mice with severe neurological symptoms were euthanized for animal welfare reasons; In the group of piscidin (10 μg/ml) + PRV, 1 mouse was heavily infected and died on day 19. And another 6 mice with severe neurological symptoms were euthanized for animal welfare reasons; In the group of piscidin (5 μg/ml) + PRV, 3 mice were heavily infected and died on day 19 and 20. And another 7 mice with severe neurological symptoms were euthanized for animal welfare reasons; In the group of piscidin (2.5 μg/ml) + PRV, 1 mice were heavily infected and died on day 20. And another 6 mice with severe neurological symptoms were euthanized respectively for animal welfare reasons; In the group of piscidin (0.5 μg/ml) + PRV, 3 mice were heavily infected and died on day 5 and 7. And another 6 mice with severe neurological symptoms were euthanized for animal welfare reasons; A total of 11 mice from all groups survived and were euthanized by the end of the experiment