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. 2019 Aug 1;19:333. doi: 10.1186/s12870-019-1889-5

Fig. 2.

Fig. 2

Representation of one α-gliadin Gli-2 locus and different mutation types potentially induced by CRISPR/Cas9. This schematic α-gliadin Gli-2 loci representation shows genes clustered and different types of mutations that can be induced by sgRNA. Simultaneous cuts in non-consecutive genes can delete the intervening genes. Similarly, two simultaneous cuts flanking the epitope can delete only this region, whilst simple small indels or base substitutions can also occur.