Fig. 2.
Representation of one α-gliadin Gli-2 locus and different mutation types potentially induced by CRISPR/Cas9. This schematic α-gliadin Gli-2 loci representation shows genes clustered and different types of mutations that can be induced by sgRNA. Simultaneous cuts in non-consecutive genes can delete the intervening genes. Similarly, two simultaneous cuts flanking the epitope can delete only this region, whilst simple small indels or base substitutions can also occur.