Table 3.
Summary of T1 Fielder-CRISPR grains, and associated T0 plants, with modified gliadin protein profile
| CRISPR/Cas9 constructs | T0 plants expressing Cas9 | T1 grains tested on Acid-PAGE | T0 plants giving T1 mutant grains with clear changes | T1 grains with clear changes in gliadin profile | T0 plants giving T1 mutant grains with any changes | T1 grains with any changes in gliadin profile |
|---|---|---|---|---|---|---|
|
Construct α1_sgRNA sgRNA_α87 |
38 | 325 | 3 (7.9%) | 4 (1.2%) | 3 (7.9%) | 8 (2.5%) |
|
Construct α2_sgRNA sgRNA_α213 + sgRNA_α324 |
12 | 288 | 7 (58.3%) | 13 (4.5%) | 7 (58.3%) | 19 (6.6%) |
|
Construct γ3_sgRNA sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603 |
35 | 280 | 10 (28.6%) | 10 (3.6%) | 15 (42.9%) | 23 (8.2%) |
|
Construct α2γ3_sgRNA sgRNA_α213 + sgRNA_α324 + sgRNA_γ86 + sgRNA_γ272 + sgRNA_γ603 |
32 | 256 | 3 (9.4%) | 11 (4.3%) | 11 (34.4%) | 20 (7.8%) |
| TOTAL | 117 | 1149 | 23 (19.6%) | 38 (3.3%) | 36 (30.8%) | 70 (6.1%) |
The plants and associated T1 grains tested on Acid-PAGE, as well as the number of T0 plants giving T1 mutant grains with “clear changes” or “any changes” in Acid-PAGE profiles, and the number of T1 mutant grains showing “clear changes” and “any changes”. For each construct mutations were observed. Mutations occurring in both the targeted and non-targeted gliadin families were included in “any changes” here.