Fig. 4.

PEPCKi decreases tumor growth. a Colo205 and b LS174T cells, were treated with 0–10 μM PEPCKi in reduced nutrient media and cell number determined after 3 days. N = 3 ± S.D. c Colo205, d Ls174T, e HT29, and f HCT116 cells were grown as spheroids for 3 days, treated with 15 μM PEPCKi, and spheroid size was determined after 2 days using ImageJ. N = 3 ± S.D. Scale bar = 35 μm. h Colo205 and i HT29 cells were inoculated into the flank of 6-week-old nude mice. Once tumors became palpable, mice were administered 10 mg/kg PEPCK inhibitor every other day and tumors measured for an additional 10 days. N = 6 ± SD. NS Not significant, *p < 0.05, **p < 0.01, ***p < 0.001