Fig. 5.
PEPCKi promotes bioenergetic stress. a Colo205 and b Moser cells were treated with 10 μM PEPCKi in reduced nutrient media and basal OCR measured using a XFe Seahorse Bioanalyzer. c Colo205 and d Moser cells were treated with 10 μM PEPCKi in reduced nutrient media and ATP coupled respiration measured using a XFe Seahorse Bioanalyzer. N ≥ 22 ± SEM. e–h Colo205 and Moser cells were treated with PEPCKi in low-nutrient conditions and ATP levels measured and ADP/ATP ratio determined. N ≥ 5 ± SD. i, j Colo205 and Moser cells were serum starved O/N and then treated with PEPCKi (0–10 μM) for 24 h and protein lysates analyzed via western blot *p < 0.05, **p < 0.01, ***p < 0.001