Table 1.
Summary table of the sex, type of calcium indicator (GCaMP6), number of MCH neurons and sampling parameters that were used to identify change in Ca2+ fluorescence in MCH neurons
| Mouse ID | GCaMP | Number of neurons | QW | NREM sleep | Pre-REM | REM sleep | Eat | Groom | Walk | Dig | Rear | Explore |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Number of samples | ||||||||||||
| F18 | Slow | 1 | 12 | 13 | 4 | 4 | ||||||
| F22 | Slow | 15 | 6 | 9 | 5 | 5 | 1 | 1 | 2 | 1 | 3 | 7 |
| F29 | Slow | 2 | 3 | 12 | 2 | 2 | 1 | 1 | 5 | 1 | 1 | 22 |
| F33 | Slow | 25 | 10 | 13 | 5 | 3 | 1 | 2 | 4 | 3 | 12 | |
| F35 | Slow | 22 | 9 | 14 | 9 | 5 | 2 | 1 | 4 | 3 | 15 | |
| M39 | Slow | 23 | 4 | 12 | 7 | 6 | 3 | 2 | 2 | 13 | ||
| M42 | Slow | 6 | 7 | 13 | 6 | 5 | 2 | 2 | 19 | |||
| F41 | Medium | 6 | 5 | 11 | 3 | 3 | 4 | 14 | 5 | |||
| M61 | Medium | 4 | 4 | 15 | 2 | 2 | 2 | 3 | 5 | 1 | 6 | |
| M62 | Medium | 3 | 5 | 18 | 7 | 4 | 1 | 5 | 6 | 6 | ||
| Total | 107 | 65 | 130 | 50 | 39 | 7 | 2 | 33 | 2 | 35 | 105 | |
| Mean sample length (s) | ||||||||||||
| F18 | Slow | 1 | 11.8 | 25.5 | 17.7 | 47.4 | ||||||
| F22 | Slow | 15 | 55.5 | 86.4 | 19.4 | 58.6 | 106.0 | 47.2 | 26.3 | 93.2 | 3.4 | 12.0 |
| F29 | Slow | 2 | 80.1 | 145.6 | 17.8 | 129.4 | 127.1 | 103.3 | 11.78 | 99.1 | 1.9 | 8.4 |
| F33 | Slow | 25 | 21.8 | 79.8 | 20.0 | 47.3 | 61.6 | 71.6 | 43.5 | 3.5 | 8.0 | |
| F35 | Slow | 22 | 29.9 | 100.7 | 15.5 | 66.9 | 44.2 | 58.6 | 90.8 | 3.9 | 6.1 | |
| M39 | Slow | 23 | 24.4 | 69.6 | 15.8 | 65.6 | 33.4 | 8.3 | 6.1 | 18.3 | ||
| M42 | Slow | 6 | 19.1 | 60.6 | 18.0 | 36.3 | 63.0 | 8.0 | 10.7 | |||
| F41 | Medium | 6 | 29.2 | 65.3 | 16.6 | 73.1 | 8.3 | 7.4 | 10.6 | |||
| M61 | Medium | 4 | 17.0 | 48.0 | 15.0 | 130.5 | 9 | 84.8 | 58.3 | 12 | 18.3 | |
| M62 | Medium | 3 | 36.7 | 56.5 | 14.9 | 39.7 | 15.0 | 36.8 | 13.2 | 4.8 | ||
| Mean ± SEM | 32.6 ± 6.5 | 73.8 ± 10.3 | 17.1 ± 0.6 | 69.5 ± 10.8 | 69.6 ± 15.0 | 59.1 ± 9.6 | 38.6 ± 9.0 | 96.1 ± 1.3 | 6.6 ± 1.2 | 10.8 ± 1.5 |
A total of 107 MCH neurons were recorded, but 1 neuron from mouse F35 was excluded since its average fluorescence in REM sleep was twice as high as the next data point. The mice were 171.4 ± 27.72 d old, weighed 36.04 ± 3.81 g, and were maintained on a 12 h light/dark cycle (23°C) with ad libitum access to food and water. Mature mice were examined to be able to support the weight of the single-photon miniscope (nVISTA; Inscopix). The EEG, EMG, and video recordings were used to identify clear episodes of the sleep–wake behavior noted in the first row. Not all mice engaged in the waking behaviors. During the test with novel objects, video recording was used to identify when the animals were exploring the objects. The entire bout of REM sleep was sampled as a single episode of REM sleep. The sex of the mice is identified in the first column. F, Female; M, male.