Table 2.

Total amino acid concentrations (μmol/mg protein) in extracts of cerebral cortical synaptosomes determined by HPLC^a

Metabolite	Control	synGLT-1 KO	t(df)	p
Alanine	2.32 ± 0.06	2.63 ± 0.07	3.227 (13)	0.007
Glutamate	46.2 ± 5.22	42.9 ± 3.48	0.5946 (15)	0.561
Glutamine	3.66 ± 0.44	3.32 ± 0.33	0.6301 (15)	0.538
Isoleucine	2.99 ± 0.31	2.74 ± 0.19	0.726 (15)	0.479
Leucine	2.90 ± 0.26	2.71 ± 0.17	0.6415 (15)	0.531
Serine	3.20 ± 0.29	3.12 ± 0.20	0.2406 (15)	0.813
Valine	3.03 ± 0.42	2.83 ± 0.34	0.3541 (15)	0.728

^aThis table summarizes concentrations of total (labeled and unlabeled) metabolites in extracts of synaptosomes prepared from cerebral cortex obtained by HPLC. No significant differences were found in the concentrations of any metabolite measured, excluding aspartate (see Fig. 5) and alanine. Results are presented as mean ± SEM; n = 7–10 preparations (from 4 control or 5 synGLT-1 KO mice).