Figure 5.

Immunohistochemical staining of ERα, ERβ, and GPER in the frontal, motor and occipital cortices in intact female Thy1M-GFP mice. A, Representative images showing immunostaining of GFP (green), ERα (red), and GPER (magenta) in the soma and distal apical dendrite of neocortical layer 5 neurons. GFP-positive somata have been outlined in yellow. The arrowheads indicate colocalization of immunoreactivity in some dendritic spines. B, Representative images showing the immunostaining of GFP (green), ERα (red), and ERβ (magenta) in the soma and apical dendrite of layer 5 neurons.