Figure 13.

Coexpression of Y₂R mRNA and tdTomato mRNA in Y₂R-tdTomato mouse brain. tdTomato mRNA (A) and Y₂R mRNA (B) expression in the BLA. C, Merged image demonstrates complete Y₂R mRNA coexpression in tdTomato cells. Arrows indicate coexpression. DAPI (blue) demarcates cell nuclei. Scale bar: C, 25 μm. D–F, Higher magnification of cells presented in A–C demonstrating the presence of Y₂R mRNA (green puncta) in tdTomato mRNA-containing cells. Scale bar: F, 30 μm. tdTomato (G) and Y₂R (H) gene expression in the CA3 region of the hippocampus. While many double-labeled cells are present (arrows), diffuse Y₂R labeling is also present that does not associate with tdTomato signaling (I). These panels demonstrate that tdTomato-synthesizing neurons coexpress Y₂R but that not all Y₂R mRNA-expressing neurons coexpress tdTomato. Scale bar: I, 30 μm.