Figure 6.

Effects of hydrogen peroxide on glial cell viability in WT and PK-KO cultures. At 6–7 d after reseeding, the cells were treated with 100 μm H₂O₂ for 3 h in EMEM plus d-glucose. A, LDH activity. B, Percentage of cells with the chromatin condensed or fragmented. C, Photomicrographs of TUNEL⁺ cells in WT and PK-KO from control and H₂O₂ (100 μm) treated cells. Scale bar, 30 μm. D, Percentage of TUNEL⁺ cells. Photomicrographs (E) and percentage of PI⁺ cells (F). Scale bar, 30 μm. The values express the mean ± SEM of six replicates each. Statistical analysis was performed by two-way ANOVA (the interaction between genotype and treatment was p < 0.05), followed by Bonferroni's post hoc test. ⁺⁺p < 0.01; ⁺⁺⁺p < 0.001, PK-KO versus WT cultures. *p < 0.05; ***p < 0.001, H₂O₂ treated cultures versus controls.