Figure 9.

Aged PK-KO midbrain glia is more sensitive to hydrogen peroxide treatment. Glia cultures maintained for 6 months in DMEM plus 15% FCS were used for these experiments. Before H₂O₂ treatment, the medium of the cultures (6 d after seeding) was changed to EMEM plus glucose. Mitochondrial activity (A), LDH activity (B), and GSH levels (C) in 6-month-old WT and PK-KO cultures after treatment with H₂O₂ at 50 μm for 3 h. Values are the mean ± SEM of six replicates each of two independent cultures. Statistical analysis was performed by two-way ANOVA (the interaction between genotype and treatment and genotype and aging were p < 0.05), followed by Bonferroni's post hoc test. ⁺⁺p < 0.01; ⁺⁺⁺p < 0.001, PK-KO versus WT cultures. ***p < 0.001, H₂O₂-treated cultures versus controls.