Figure 2.
Mutant TauRD protein levels in transgenic mice. Representative immunoblots probed with K9JA polyclonal antibody that recognizes the repeats plus C-terminal domain of Tau. A, Levels of Tau in cortex of mutant TauRD-inducible transgenic mice. Cortex brain lysates from nontransgenic littermate, proaggregation (TauRD/ΔK280) and antiaggregation (TauRD/ΔK280/2P) mutants at 9 months of gene expression. Ten micrograms of total protein is loaded per lane. B, Ratio of mutant TauRD versus endogenous Tau in transgenic mice at 9 months of gene expression (n = 3 mice/group) (mean ± SEM, 0.73 ± 0.02 for the proaggregation mutant; 0.68 ± 0.07 for the antiaggregation mutant). C, Immunoblots of Sarkosyl-soluble and -insoluble fractions from cortex lysates of proaggregation and antiaggregation mutant mice at 3 and 12 months of gene expression. Blot analysis with K9JA antibody. Sarkosyl-soluble fractions show the presence of endogenous (∼55 kDa) and exogenous (∼12–14 kDa) Tau protein in the case of proaggregation and antiaggregation mutants. The Sarkosyl-insoluble fractions indicate the aggregation of endogenous mouse Tau and exogenous mutant TauRD in the proaggregation mutant mice, but the absence of aggregation in the antiaggregation mutant mice.