Figure 5.
Phosphorylation at KXGS motifs and mislocalization of TauRD/ΔK280 to the somatodendritic compartment. A, Bar diagram of Tau repeat domain and 12E8 epitopes, the phosphorylation sites of MARK. B, Somatic relocalization of hyperphosphorylated TauRD/ΔK280 in hippocampal, CA1 neurons at 2 months after gene expression of the proaggregation mutant. C, Absence of hyperphosphorylated TauRD/ΔK280/2P in hippocampal CA1 region at 2 months after gene expression of the antiaggregation mutant. D, Immunohistochemistry of proaggregation mouse brains at 12 months of TauRD/ΔK280 gene expression showed phosphorylation and translocation of Tau protein in the somatodendritic compartment of subiculum and (E) only mild phosphorylation of Tau protein in the antiaggregation mutant at the same time point. Scale bar: B–E, 20 μm.