Figure 2.
NGF does not affect cold sensitivity of nodose ganglion neurons. A, Simultaneous recording of [Ca2+]i (top trace) and bath temperature (bottom trace) during application of a cooling ramp in four representative examples of cultured nodose neurons under different experimental conditions. Insets, Pie charts with the percentage of CS and CI neurons under each condition. B, Mean [Ca2+]i elevation during cooling in acutely cultured (2, 6, and 24 h without NGF and 24 h with 20 ng/ml NGF). C, Correlation between [Ca2+]i elevation to high K+ (30 mm) application and to a cooling pulse in individual neurons. *p < 0.05.