Figure 7.

Later SCLIP inhibition blocks PC dendrite elongation and branching at 14 div. A, Examples of PC morphologies observed at 14 div after infection with Lv-shSCLIP at 7 div. Cerebellar organotypic cultures were infected at 7 div and immunostained for GFP (green) and CaBP (red) 7 d later. Whereas untransduced PCs (red) are typically in stages 3 (a) and 4 (b), PCs transduced with Lv-shSCLIP (green) mostly display several dendritic processes or a single and thick trunk without branches or spines, respectively defined as branchless stage 3 or branchless stage 4. Scale bar, 20 μm. B, Quantified results of three combined independent experiments illustrating the effect of later SCLIP inhibition on PC dendritic differentiation stages at 14 div. In contrast to PCs transduced with Lv-shCtrl, which are mostly in stages 3 and 4, ∼35 and 30% of PCs transduced with Lv-shSCLIP are in a branchless stage 3 or branchless stage 4, respectively. The remaining PCs exhibit a round or fingered morphology as that observed at 14 div after early RNAi. Values shown are mean ± SEM.