Single-channel properties of HCLA channels. Left, Sample single-channel recordings from HCLA homomers (from hclA-transfected S2 cells) and native HA receptors (from Drosophila LMCs), recorded at the holding potentials indicated, with 10 μm HA in the recording pipette in both cases. At least 5000 transitions from each recording were idealized with SCAN. Middle, Amplitude (Amp) distributions of time course-fitted opening transitions, generated and fitted with multiple Gaussians by maximum likelihood with EKDIST (fitted amplitudes and relative contributions of subconductance states are indicated). Right, Open time distributions of fitted transitions, generated and fitted by multiple exponentials with EKDIST. Fitted time constants and relative contributions are indicated in insets. A 100 μs time resolution was imposed for all distributions.