Figure 7.

Expression profile of hclA and hclB determined by mRNA tagging. Shown is a RT-PCR gel using primers for hclA and hclB, as well as elav and repo controls (to identify neurons and glia, respectively) and rpl32 (“housekeeping” control). All transcripts were detected in whole-brain tissue using conventional RT-PCR (top row). mRNA immunoprecipitated from all neurons using elav-Gal4 × UAS-hPF included both hclA and hclB transcripts, as well as elav (positive control); the lack of repo signal (negative control) confirms that there was no contaminating signal. Glial mRNA (using repo-gal4) contained hclB (and repo) but no hclA (or elav). In the LMCs (L2-Gal4), only hclA and elav were detected. All samples expressed the general housekeeping control gene rpl32 (ribosomal protein). ntc, No template control; m, markers.