Table 4.
Single-channel characteristics (conductance levels and relative areas) for recombinant HCLA homomers and native HA receptors from LMCs at different holding potentials
| Receptor | Vm (mV) | γ (pS) | A (%) |
|---|---|---|---|
| HCLA homomeric | −100a | 25.9 ± 8.4 | 20 |
| 43.9 ± 6.4 | 21 | ||
| 59.9 ± 6.5 | 59 | ||
| +80b | 24.9 ± 10.0 | 35 | |
| 39.2 ± 4.2 | 65M | ||
| LMC native | −80 | 16.8 ± 5.5 | 21 |
| 35.5 ± 10.6 | 32 | ||
| 54.6 ± 7.4 | 47 | ||
| +80 | 23.0 ± 8.1 | 13 | |
| 39.3 ± 4.3 | 87 |
The data from the native receptor are based on a single exemplary recording (10 μm HA). Data from five further native patches showed similar behavior with a major conductance state of 50–60 pS at −80 mV and clear indications of subconductance states, but recording quality was not suitable for more rigorous analysis. Data are based on >1500 channel transitions per patch (after imposition of 100 μs resolution). γ, Conductance levels; A, relative areas; Vm, holding potential. an = 4 patches. bn = 2 patches.