Figure 3.
Summary of the in vivo quantification of GECI and OGB-1 fluorescence changes at steady state. A, B, Side-by-side comparison of maximum fractional fluorescence changes (A) and SNR (B) plotted as a function of Δ[Ca2+]i (logarithmic x- and y-axes). C, Δ[Ca2+]i necessary to elicit a fractional fluorescence change with SNR of 2 under our recording conditions. D, Time constants for the decay of the fluorescence change, determined after 40 Hz stimulation (20 Hz for YC2.60, D3cpv, and OGB-1). Parameters in A–C were analyzed at steady state; OGB-1 data are from Figure 1, and GECI data are from Figure 2.