Figure 5.

Connexin 36 and connexin 43 protein expression levels are upregulated in cold stressed rats. Connexin expression levels were measured by Western blot. A, Representative immunoblots showing specific detection of Cx36 and Cx43 in the adrenal medulla of control and stressed rats. Actin was used as internal loading control. B, Histogram summarizing the densitometric analysis of immunoblots (normalized by actin) from seven control and seven stressed rats. Quantitative analysis shows that Cx36 and Cx43 expression was significantly increased in stressed rats (3- and 2-fold increase, respectively). *p < 0.01, compared with unstressed rats. ctrl, Control. The number of rats used for each condition is indicated in parentheses.