Figure 5.
Purkinje-cell-specific expression of Kv3.3 rescues motor task performance. A, During the 5 d test on the 1 cm beam, Kcnc3-single mutants (SKO) perform worse than wild-type (p < 10−6) and rescue mice (repeated-measures ANOVA, p < 10−4). Although rescue mice perform better than Kcnc3-null mutants do, their performance is worse than that of wild type (repeated-measures ANOVA, p = 0.026) during the 5 d test, probably because of the impaired beam performance on day 1 (ANOVA; wt/rescue, p = 0.051). Importantly, all genotypes significantly improve their performance during the 5 d test (repeated-measures ANOVA, p < 10−6). Significant differences between SKO and wild-type or rescue mice for each day are indicated (ANOVA, *p < 0.05, **p < 0.01, ***p < 0.001). B, Mice carrying three Kcnc-null alleles [i.e., heterozygous Kv3.1/homozygous Kv3.3 mutants (+/−; −/−)] perform worse than +/+; +/− controls (repeated-measures ANOVA, p < 10−6) and rescue mice on the +/−; −/− background (p = 0.014). Although rescue mice perform better than +/−; −/− mutants, their performance appears worse than that of controls (p = 0.070). Importantly, all genotypes significantly improve their performance during the 5 d test (repeated-measures ANOVA, p < 10−6). Significant differences between +/−; −/− mice and +/+; +/− or rescue mice for each day are indicated (ANOVA, **p < 0.01, ***p < 0.001). Error bars indicate SEM.