Figure 2.

Glutamate-induced axon retraction requires non-NMDA receptor activity and intracellular calcium dynamics. A, Representative time-lapse images of the axon terminal of a neuron whose soma received glutamate application in the bath of 20 μm CNQX. B, Summary data of perisomatic glutamate application in the presence of CNQX. Data represent means ± SEM of eight axons. C, Local perisomatic application of AMPA (300 μm in pipette) (circle) (n = 9), but not NMDA (1 mm in pipette) (square) (n = 6) or ACPD (1 mm in pipette) (triangle) (n = 5), induced axon retraction. D, Bath application of 10 μm nifedipine (circle) (n = 7), 1 μm thapsigargin (square) (n = 12), and 100 μm 2APB prevented the axon retraction (triangle) (n = 6). *p < 0.05 versus 0 min, paired t test.