Figure 6.

Akt activity is increased in cultured astrocytes in Pax6-deficient condition. A, The equal amount of total protein (10 μg) from cell lysates of cultured WT and Sey/Sey astrocytes (Fig. 2A) are subjected to Western blot analysis with antibodies against Akt, pAkt, or Hsp90. The level of expression of pAkt is higher in Sey/Sey astrocytes than in the WT astrocytes. B, Relative expression level of Akt and pAkt protein normalized by Hsp90. The expression level of Akt protein in WT astrocytes is similar that in Sey/Sey astrocytes. In contrast, the expression level of pAkt protein in Sey/Sey astrocytes is 1.8-fold higher than that in the WT astrocytes (p = 0.00003). The results are presented as the mean values ± SD of the samples (n = 5; ***p < 0.0001).