Figure 2.
The reduced slow AHP is not caused by alterations in the underlying K+ channels. Whole-cell recordings were obtained using electrodes filled with internal solution including 100 μm Fluo-3 and 10 mm DM-nitrophen (Ca2+ cage). A, B, Prnp0/0 neurons filled with this solution displayed a significantly reduced action potential evoked slow AHP (A, left, gray traces), associated with a significant attenuation of Ca2+ increases (B, left, gray traces). In contrast, flash photolysis evoked a robust and similar AHP in both Prnp +/+ and Prnp0/0 neurons (A, right), associated with similar Ca2+ transients in the two genotypes (B, right). *p < 0.05.