Figure 6.

Block of SERCA by thapsigargin abolishes the observed changes in slow AHP and Ca²⁺ signaling Prnp^0/0 neurons. A, Bath application of thapsigargin (3 μm) increased slow AHP amplitude (top), peak Ca²⁺ levels (middle), and basal Ca²⁺ levels in Prnp^0/0 neurons (open circles), but not Prnp ^+/+ neurons (filled circles). B, C, Representative traces of voltage response (B) and Ca²⁺ changes (C) evoked by 50 action potentials at 20 Hz; control responses are shown in the left panel, thapsigargin treated responses are illustrated in the right panel (Prnp^0/0 in gray; Prnp ^+/+ in black). *p < 0.05; **p < 0.01; ***p < 0.005.