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. 2008 Jan 9;28(2):434–446. doi: 10.1523/JNEUROSCI.4374-07.2008

Figure 2.

Figure 2.

Inducible deletion of Smad4 in adult astrocytes and neural stem cells in the adult SEZ. A, B, In situ hybridization of Smad4 mRNA in the adult SEZ 10 d after activation of CreERT2 expressed in the GLAST locus (GLAST:CreERT2) by tamoxifen in mice without (A) or with (B) exon 8 of Smad4 flanked by loxP sites. Note the loss of Smad4 mRNA in B versus A. C, D, Fluorescent micrographs of p-Smad1/5/8 immunostaining in GLAST:CreERT2 mice WT for the Smad4 locus (C) or homozygous for the floxed exon 8 of Smad4 (D) 10 d after induction of Cre-mediated recombination by tamoxifen. Note the widespread loss of nuclear localization of p-Smad1/5/8 after deletion of Smad4 (D). The arrows in D indicate some of the remaining p-Smad1/5/8+ cells also demonstrating the same quality of immunostaining. LV, Lateral ventricle; D, dorsal; V, ventral; R, rostral; C, caudal. Scale bars: (in B) A, B, 100 μm; (in D) C, D, 100 μm.