Figure 3.

Spontaneous NMDA mEPSCs are decreased after inhibition of DNMTs. A, Sample traces of whole-cell voltage-clamp recordings of NMDA mEPSCs done in the presence of CNQX, PTX, strychnine, glycine, and TTX. AP-5 was then washed on to determine baseline noise levels. B, Bar graph demonstrating that both 5azaC and Zeb treatments resulted in significant decreases in the amount of mEPSC charge (Q) per 10 s intervals divided by the baseline amount of Q after AP-5 was added. **p < 0.01; ***p < 0.001. C, Sample traces of evoked NMDA EPSCs recorded from treated neurons. D, Bar graph showing no changes in average EPSC amplitudes after treatment with DNMT inhibitors. The numbers on the bars indicate the number of experiments (B, D).