Figure 3.

Re-introduction of PS1 into PS1−/− neurons restores PI3K/Akt signaling and suppresses neuronal degeneration. A, PS1−/− primary neurons were infected with either HSV-vector virus (V), or HSV-PS1 (PS1) recombinant virus on their 10th day in culture. Lysates were analyzed as indicated. B, Statistical analysis of the results from four independent experiments. C, MAP2 (red) and DAPI (blue) staining of 13 DIV PS1−/− mouse primary neurons, infected with EGFP-expressing (green) HSV-EGFP-vector and HSV-EGFP-PS1 recombinant viruses.