Figure 4.

PS1 rescues via PI3K/Akt signaling. A, PS1−/− primary neurons, infected with either HSV-vector virus (V), or HSV-PS1 (PS1) recombinant viruses were incubated in the absence (NT) or presence of LY294002 (LY) or the γ-secretase inhibitor l-685,458 (γ-inh). Lysates were analyzed as indicated. B, Lysates from PS1−/−, PS1+/+ and PS1+/− primary neurons, infected with HSV-EGFP-vector, HSV-EGFP-constitutively active Akt or HSV-EGFP dominant-negative Akt(DN) viruses were analyzed as indicated. C, MAP2 (red) and DAPI (blue) staining of 14 DIV PS1−/− mouse primary neurons, infected with EGFP-expressing (green) HSV-EGFP-vector and HSV-EGFP CA-Akt recombinant viruses.