Figure 1.

Generation of transgenic mice with forebrain expression of DAT (CamDAT mice). A, Inducible forebrain DAT expression mice were generated by breeding forebrain-specific CaMKIIα-tTA mice and tetO-DAT mice. B, DAT mRNA expression was detected in the striatum (STR), cerebral cortex (CTX), hippocampus (HIP), olfactory bulb (not shown), and olfactory tubercle (not shown). Endogenous DAT mRNA was detected in SNc. In control mice (CTL), no DAT mRNA expression was detected in the forebrain. C, DAT proteins were detected in regions with mRNA expression as well as in globus pallidus (arrow) and substantia nigra pars reticulata (arrowhead) that are main striatum efferent nuclei. Top, Forebrain region shown in more rostral sections. Bottom, Midbrain region shown in more caudal sections.