Figure 5.

Oxidative stress in transgenic mice. A, Oxidation-induced protein modification by dopamine. Using HPLC, we found significant increases of cys-dopamine and cys-DOPAC in CamDAT mice after 3 weeks of DOX withdrawal. Similar increases were found in cerebral cortex. In contrast, no change was found in the cerebellum (n = 8 per group, unpaired t test, ***p < 0.001). B, Decreased glutathione content in brain tissues. Total glutathione (GSH+GSSG), GSH, and GSSG were 26% (p < 0.01), 29% (p < 0.01), and 27% (p < 0.05) lower, respectively, in striatum of CamDAT mice after 4 weeks of DOX withdrawal compared with control mice (n = 5 per group, unpaired t test, *p < 0.05, **p < 0.01). No change was found either in the cortex or cerebellum (p > 0.05, n = 5 per group).