Figure 1.

Generation of DAL mice. A, Schematic drawing of the transfected DNA fragment. DAL mice were generated by the injection of 4.2 kb ApaLI–PvuI fragment into fertilized eggs. B, RT-PCR analysis revealed that three transgenic founders expressed exogenous ALDH2*2 in the brain. For PCR, primers shown in A were used (see Materials and Methods). The obtained PCR fragments (N) were digested with AclI (D). Two short fragments (54 and 30 bp) were derived from exogenous ALDH2*2, whereas the nondigested fragment was from endogenous ALDH2. *Nonspecific bands. C, In the brain of DAL101, real-time RT-PCR analysis revealed more exogenous ALDH2*2 transcripts relative to endogenous ALDH2 transcripts in the olfactory bulb, cortex, hippocampus, and midbrain. Data are mean ± SEM (n = 4).