Figure 2.

NR2B M3 contains an ER retention signal. A, COS7 cells transfected with GFP-NR2B truncations were stained for surface GFP. Schematic diagrams show the structure of GFP-NR2B truncation constructs. B, The percentages of cells having surface staining were determined for >300 GFP-expressing cells for each construct in three experiments. The bar graph represents mean ± SEM. C, Data represent mean ± SEM of fluorescent intensities per unit area obtained for surface (black) or total GFP (white) expression. More than 45 GFP-expressing cells for each construct were analyzed in three experiments. **p < 0.001, ANOVA.