Figure 6.

NR1 M4 is necessary for masking the NR2B M3 ER retention signal. A, Live cells transfected with indicated combinations of NR1 and NR2B constructs were immunostained with anti-GFP antibodies. Schematic drawings of membrane topology of NR1 (gray) and NR2B (black) subunits are shown. B, Data represent the percentages of surface labeled cells expressing indicated NMDA receptor subunits (in mean ± SEM; n >300 cells for each combination of cDNAs in three experiments). C, Each vertical bar represents the mean ± SEM of fluorescent intensities per unit area (n >45 cells for each combination of NMDA receptor subunits in three experiments). **p < 0.001, ANOVA.