Figure 8.

The replacement of NR1 M4 for AchR α M3 inhibits surface targeting of the NMDA receptors. A, The amino acid sequences of the NR1 M4 and AchR α M3 are shown. B, COS7 cells transfected with indicated combinations of cDNAs were immunostained for surface YFP. Schematic diagrams of membrane topology of NR2B (black) and NR1 (gray) subunits with replaced M4 for AchR α M3 (white) are illustrated. C, Bar graph represents the percentages of YFP-expressing COS7 cells exhibiting the surface staining in mean ± SEM; n >300 cells in three experiments. D, Measurements of cell surface (black) and total (white) expressions of indicated NMDA receptor subunit variants. Each vertical bar represents the mean ± SEM of fluorescent intensities per unit area; n >45 cells in three experiments. **p < 0.001, ANOVA.