Figure 5.
The tonic biosynthesis of 3α5α-NS is regulated during postnatal development. A, In young animals (<P8), τdecay values were elevated and similar in lamina II (open circles) and laminas III–IV (filled circles) neurons. At P9–P15, τdecay was significantly decreased in laminas III–IV but remained elevated in lamina II neurons. At >P21, τdecay was decreased in lamina II neurons reaching values similar to that in lamina III–IV neurons. Two-way ANOVA, with factors age and lamina, showed a significant interaction (F(2,62) = 5.63; p < 0.01). B, Incubation of spinal cord slices for >5 h with PK11195 (10 μm; gray bars) or >3 h with finasteride (50 μm; black bars) significantly reduced τdecay values (white bars) in both lamina II and laminas III–IV at <P8 but only in lamina II at P9–P15. Both substances had no significant effect at >P21, neither in lamina II nor in laminas III–IV. One-way ANOVA was used for factor treatment for each lamina at each age.