Figure 9.

Spike timing-dependent Ca²⁺ influx. A, Time courses of Ca²⁺-bound Ca²⁺ indicator in cytosol (Conc, 100 μm; Kd, 580 nm) by prespiking → postspiking (T_post − T_pre = 4 ms; left) and postspiking → prespiking (−4 ms; center). Time courses of Ca²⁺-bound Ca²⁺ indicator by a prespike alone were overlaid. Maximum of Ca²⁺-bound Ca²⁺ indicator by prespiking, prespiking → postspiking and postspiking → prespiking are shown (right). B, Time courses of [Ca²⁺]_PSD and [Ca²⁺]_cytosol by prespiking → postspiking with the indicated timing.