Figure 5.

CCL5/RANTES in ACM maintained proper neuronal activity. Primary neurons (at 4 DIV) cultured in the indicated ACM containing the control IgG (0.2 μg/ml), the anti-CCL5/RANTES IgG (0.2 μg/ml), or the recombinant CCL5/RANTES (450 ng/ml) as indicated for 4 d. Cells were loaded with fura-2 (5 μm) and stimulated with 60 mm K⁺ (marked by the arrow). A, Representative traces depict the [Ca²⁺]_i elevation evoked by the high external K⁺ as indicated. B, The maximal calcium response after depolarization was quantified using Metafluor software. At least 100 cells for each condition were analyzed. Values are expressed as percentages of the calcium in WT astrocytes and represent the mean ± SEM of four independent experiments. ^ap < 0.05, compared with WT-ACM containing the control IgG; ^bp < 0.05, compared with R6/2-ACM containing the control IgG, by Student's t test followed by the Mann–Whitney rank sum test.