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. 2008 May 14;28(20):5369–5382. doi: 10.1523/JNEUROSCI.4738-07.2008

Figure 3.

Figure 3.

In vitro CaMKII phosphorylation sites of Homer3 were at Ser120, Ser159, and Ser176, and MAPK site was at Ser141. A, Wild-type or mutated Homer3 protein was processed for in vitro phosphorylation by CaMKII, and the phosphate incorporation was analyzed by autoradiography. B, Wild-type or mutated Homer3 protein was processed for in vitro phosphorylation by MAPK, and the phosphate incorporation was analyzed by autoradiography. C, Schematic diagram of phosphorylation sites of Homer3 by CaMKII (red S) and MAPK (blue S). D, Homer3 phosphorylation sites were conserved among species. Each amino acid sequence was from GenBank accession numbers NP_036114 (mouse), NP_445762 (rat), NP_004829 (human), XP_418233 (chicken), XP_687581 (zebra fish), or NP_001080624 (Xenopus).