Figure 2.

Effects of KCl of differentiation of neurospheres. A, The primary neurospheres in low KCl conditions contained only astrocytes after differentiation. B, However, after repeated passaging in vitro, the neurospheres from the high KCl-stimulated cultures contained a large number of βIII-tubulin-positive neurons (red) and GFAP-positive astrocytes (green). C, Passaged neurospheres were also stained for nestin (red), a marker for stem cells, and GFAP (green) and (D) doublecortin (green), an early neuronal marker. Cell nuclei are counterstained with DAPI (blue). Magnification: A–D, 20×; D, inset, 40×.