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Expression of P2Y12 protein in microglia in the dorsal horn 7d after PSNL with immunohistochemical analysis. P2Y12 immunoreactivity was increased on the side ipsilateral to the PSNL compared with the contralateral side (A, B). Double-immunofluorescent histochemistry (C–H) in both sides showed that P2Y12 protein (red; C, F) colocalized with Iba1-labeled cells (green; D, G). Merged images are E, H. Scale bars: A, B, 200 μm; C–H, 50 μm. contra, Contralateral; ipsi, ipsilateral.
