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. 2008 Mar 12;28(11):2820–2826. doi: 10.1523/JNEUROSCI.4145-07.2008

Figure 6.

Figure 6.

GlyT2-EGFP-expressing Golgi cell dendrites are restricted at zebrin II+/− parasagittal stripe boundaries. Deconvolution analysis of a 200 μm stained section cut transversely through lobule VIII of the adult GlyT2-EGFP mouse cerebellum and immunofluorescence labeled for zebrin II. The Golgi cell dendrites (red) respect the zebrin II+/− Purkinje cell boundary (green; arrows). In the three-dimensional (3-D) rendered image, the GlyT2-EGFP+ Golgi cell dendrite does not invade the zebrin II+ stripe. The 3-D image has been rotated to better appreciate the relationship between the dendrites and Purkinje cells. The green arrow (x) represents the longitudinal plane (the arrowhead indicates the right side). The red arrow (y) shows the top and bottom position (the arrowhead indicates the bottom). The inset is a single slice taken from the same experimental slide acquired by normal stack microscopy demonstrating the surface of the tissue; the Golgi cell dendrite can be traced from deep within the granular layer into the molecular layer, where it tracks along the zebrin II +/− border. The positions of the neighboring zebrin II− Purkinje cell somata are outlined with dotted lines. ml, Molecular layer; pcl, Purkinje cell layer; gcl, granule cell layer; GC, Golgi cell; PC, Purkinje cell. Scale bars, 50 μm.