Skip to main content
. 2008 Mar 19;28(12):3051–3059. doi: 10.1523/JNEUROSCI.5620-07.2008

Figure 1.

Figure 1.

Characterization of 3xTg-AD/Bcl-2-OE mice. A, 3xTg-AD mice were bred to Bcl-2-OE mice to generate F1 founder mice. 3xTg-AD/Bcl-2-OE mice from this cross were than backcrossed with 3xTg-AD mice for the purpose of generating F2 founder mice. The presence of the Bcl-2 transgene of F2 mice was confirmed by PCR using primer sequences for the human Bcl-2 gene (arrow). As depicted, mice 1–4 and 8 were identified as being positive for the human Bcl-2 transgene. B, Representative immunofluorescence labeling in an 18-month-old 3xTg-AD/Bcl-2-OE mouse showing the cytoplasmic expression of Bcl-2 in the cortex. Bottom left, Bcl-2 staining (green) with nuclear staining using Hoechst (blue). Bottom right, Western blot analysis confirming the presence of the human Bcl-2 protein.