Figure 3.

Inhibition of P2Y₁₂R prevented the development of tactile allodynia. A, The PWT of the ipsilateral hindpaw in response to tactile stimulation in rats were examined before nerve injury and 1, 3, 5, and 7 d after nerve injury. Rats were subjected to intrathecal administration of AR-C69931MX (ARC) at several doses (n = 5 each) or vehicle (n = 7), twice a day, after peripheral nerve injury. B, PWTs were measured in the contralateral hindpaws. Each data point represents the mean ± SEM of PWT (*p < 0.05 vs vehicle group; ^#p < 0.05 vs premeasurement by multiple comparison by Tukey's test after a repeated-measure two-way ANOVA). C–F, L5 spinal cord segment from 7 d postoperative rats that received intrathecal injections of vehicle (PBS; C, D) or AR-C69931MX (50 pmol; E, F) twice daily were subjected to immunohistochemistry using anti-OX-42 antibody. Scale bar, 100 μm.